FIGURE 1. Expression of hif-1 during Xenopus development. A, predicted domains of Xenopus HIF-1 . bHLH, basic helix loop helix binding domain; PAS, Per-Arnt-Sim domain; TAD-N, N-terminal activation domain; TAD-C, C-terminal activation domain. B, temporal expression profile of the hif-1 mRNA. RT-PCR analysis was carried out from stage 1 to 25 using a primer set for hif-1 . Quality of RNA at each stage was assessed by amplification of the ubiquitous transcript, ornithine decarboxylase (odc). C, hif-1 expression was examined by whole mount in situ hybridization of embryos at the stage indicated in each panel. a, lateral view of stage 23 embryo; b, lateral view of stage 28 embryo; câ e, Stage 33/34 embryos, lateral view (c), ventral view (d), and higher magnification image (e) of the anterior portion seen in panel d. hif-1 expression in the eyes (yellow arrowhead), brain (yellow arrow), kidneys (red arrow), somites (yellow asterisk), branchial arches (black arrowhead), myocardium (red arrowhead), and the ventral blood island (VBI, black asterisk). f, transversal section of a stage 33/34 embryo at the white line in panel d reveals hif-1 expressed in myocardium.
Image published in: Nagao K et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hif1a.L | hif-1alpha, hif1alpha, LOC445838, xhif1a | X. laevis | Sometime during NF stage 23 to NF stage 33 and 34 | somite otic vesicle pronephric kidney pronephric nephron pronephric tubule eye |
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