Figure 4. Notum Expression Patterns during Xenopus Embryogenesis (A and B) RT-PCR revealed that Notum is maternally and zygotically expressed throughout Xenopus embryogenesis (A), and is enriched animally and dorsally at stage 10.5; animal caps, AC; dorsal marginal zone, DMZ; ventral marginal zone, VMZ; vegetal caps, VC; a pan-mesodermal marker, Xbra; a dorsal marker, Chordin; an animal and ventral marker, Msx1. (CâT) Whole mount in situ hybridization for Notum/Notumâ expression, showing lateral view at 4-cell and stage 6.5 (C and D); lateral and bisected view at stage 8.5 (E and F); bisected view at stage 9.5 (G); lateral and bisected view at stage 10.5, with dorsal on right (H and I); dorsal and bisected view at the stage 11 (J and K); with an enlarged anterior view showing stronger expression anteriorly (L); dorso-anterior (M); dorsal (N, anterior on top); and cross-section (O, dorsal on top; and P, an enlarged view) at stage 15; lateral view at the tail bud stage (Q); with an enlarged anterior view indicating expression in the cement gland (R, arrow); lateral view at the early tadpole stage (S); and with an enlarged view indicating expression in the pronephros region (T, arrowhead). The blue color at the blastocoel surface in bisections was non-specific. See also Figure S3.
Image published in: Zhang X et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| notum.L | X. laevis | Throughout NF stage 11 | neuroectoderm anterior mesoderm | |
| notum.L | X. laevis | Throughout NF stage 13 | neural plate | |
| notum.L | X. laevis | Throughout NF stage 15 | pre-chordal neural plate border epidermis non-neural ectoderm lateral plate mesoderm | |
| notum.L | X. laevis | Throughout NF stage 25 | cement gland | |
| notum.L | X. laevis | Throughout NF stage 3 (4-cell) | animal hemisphere animal pole | |
| notum.L | X. laevis | Throughout NF stage 35 and 36 | cement gland pronephric kidney otic vesicle pharyngeal arch branchial arch hyoid arch head head mesenchyme | |
| notum.L | X. laevis | Throughout NF stage 6.5 | animal hemisphere animal pole | |
| notum.L | X. laevis | Throughout NF stage 8 to NF stage 10.5 | dorsal marginal zone ventral marginal zone |
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