Fig. S4. Temporal and spatial expression pattern of Custos in Xenopus embryo. (A) Xenopus Custos is expressed throughout development. The expression level of Custos RNA was assessed by RT-PCR analysis. Ornithine Decarboxylase (ODC) was used as an internal control, RT(â) is without reverse transcriptase as a negative control. (B) Custos is highly expressed in the anterior region of the embryo. Whole-mount in situ hybridization was performed using a Xenopus Custos anti-sense RNA probe. No signal was detected using a Custos sense RNA probe. (C) Expression of Custos does not induce or suppress levels of activated RhoA in HEK-293T cells; the GEF Ephexin is used as a positive control. (D) Sequence of Xenopus Custos antisense morpholino oligonucleotide (MO) is indicated by red letters.
Image published in: Komiya Y et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 12 | ectoderm neuroectoderm non-neural ectoderm |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 15 | neuroectoderm neural plate pre-chordal neural plate chordal neural plate neural plate border neural crest premigratory neural crest cell |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 20 | neural plate pre-chordal neural plate chordal neural plate neural plate border optic vesicle neural crest cranial neural crest anterior placodal area |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 23 | neural tube posterior neural tube anterior neural tube dorsolateral neural tube ventrolateral neural tube neural crest cranial neural crest optic vesicle anterior placodal area |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 29 and 30 | eye optic vesicle otic vesicle neural crest cranial neural crest branchial arch mandibular crest hyoid crest mandibular arch hyoid arch spinal cord brain forebrain midbrain hindbrain |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 35 and 36 | spinal cord otic vesicle brain forebrain midbrain hindbrain eye head mesenchyme neural crest cranial neural crest |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout NF stage 9 | animal hemisphere |
| c1h12orf43.L | c12orf43, custos | X. laevis | Throughout unfertilized egg stage | egg |
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