Supplemental Figure 1: in situ hybridization of DHFR in Xenopus laevis. A) Antisense probe targeting DHFR revealed expression in the neural tube, future eyes and face surrounding the presumptive embryonic mouth in stages 22-30. B) The sense probe was devoid of staining. C) Antisense probe targeting reduced folate carrier (RFC) revealed overlapping expression domains with DHFR, however, DHFR appeared to be more ubiquitously expressed than this gene. These results suggest the possibility that reduced folate carrier is only necessary in some cells and could also perform different/additional functions than simply transporting folate into the cell.
Image published in: Wahl SE et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| dhfr.L | dihydrofolate reductase | X. laevis | Throughout NF stage 22 | neural tube optic vesicle posterior |
| dhfr.L | dihydrofolate reductase | X. laevis | Throughout NF stage 26 | eye posterior pharyngeal arch |
| dhfr.L | dihydrofolate reductase | X. laevis | Throughout NF stage 29 and 30 | central nervous system brain spinal cord |
| slc19a1.L | chmd, folt, ifc1, LOC108701405, refc, RFC, rfc1, XRFC | X. laevis | Throughout NF stage 29 and 30 | central nervous system brain spinal cord pharyngeal arch mandibular arch hyoid arch branchial arch |
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