Fig. 1. Pattern of hsp30 mRNA accumulation during early Xenopus development. Whole mount in situ hybridization with DIG-labeled hsp30 antisense riboprobe was carried out with control (A) and heat shocked (B) (1 h at 33 â¦C) Xenopus midtailbud stage embryos. Preferential heat-induced accumulation of hsp30 mRNA was observed in lens placode (LP), somites (S), proctodeum (P) and cement gland (CG). Constitutive hsp30 mRNA accumulation was also observed in cement gland. (C) Treatment of Xenopus embryos with HDIs resulted in premature heat-induced accumulation of hsp30 mRNA at the gastrula stage rather than at the late neurulaâearly tailbud stages as found in non-HDI-treated embryos. HDI treatment enhanced heat-induced hsp30 mRNA accumulation in neurula and tailbud embryos. These experiments suggest that hsp30 gene expression during development is regulated at the level of chromatin structure. Xenopus embryos were incubated with (+) or without (â) 30 nM trichostatin A (TSA), 5mM valproic acid (VPA), or 5mM sodium butyrate (NaB). Total RNA was isolated from control (C; 22 â¦C) and heat shocked (H; 1 h at 33 â¦C) embryos and subjected to Northern blot analysis using an hsp30 antisense riboprobe. Arrows indicate the positions of hsp30 mRNA.
Image published in: Heikkila JJ (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hsp30e.L | X. laevis | Throughout NF stage 28 | lens placode proctodeum cement gland somite epaxial muscle |
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