Fig. 4. xSOUL is a novel secreted protein of the SOUL/Heme-binding protein family. (A) Culture medium of 293T cells transfected with green fluorescent protein cDNA as control ( GFP) or Xenopus SOUL cDNA (xSOUL ). xSOUL is secreted as a 23 kD protein. (B) Evolutionary relationship of xSOUL and other members of the SOUL/heme binding protein (HBP) family. Proteins with the greatest sequence similarity cluster together and branch lengths are proportional to distance (TreeTop-Phylogenetic Tree Prediction; http://www.genebee.msu.su/services/ phtree_reduced.html). Only the mature proteins have been considered. Note that xSOUL is most closely related to a derived family member in the plant Arabidopsis thaliana (aSOUL). The listed proteins have the following GenBank accession numbers: xSOUL (GenBank accession number DQ004682); aSOUL (NM101570); human SOUL (AF117616); mouse SOUL (AF117614); zebrafish SOUL (BC045936); human HBP (AF117615); mouse HBP (AF117613); chick SOUL (AF117612). (C) Comparison of xSOUL with other SOUL/HBP sequences. Note that only xSOUL and aSOUL have cleavable signal peptides (triangle). The putative hemebinding region as reported by Zylka and Reppert (1999) is overlined. (D) Four-cell stage embryo in animal view showing maternal expression of xSOUL. (E) Anterior view of advanced neurula. Note weak expression in the anterior brain (arrowhead). (F) Tailbud stage embryo with distinct expression in the forebrain, lens, somites and gut.
Image published in: Pera EM et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hebp2.L | pp23, soul | X. laevis | Throughout NF stage 20 | anterior neural tube |
| hebp2.L | pp23, soul | X. laevis | Throughout NF stage 29 and 30 | forebrain endoderm alimentary system somite eye lens cranial placode |
| hebp2.L | pp23, soul | X. laevis | Throughout NF stage 3 (4-cell) | animal blastomere animal animal pole |
Image source: Published
Permanent Image Page
Printer Friendly View
XB-IMG-154153