Fig. 1. NC cells express both PDGF-A and PDGFRα. (A-I) Whole-mount in situ hybridization of Xenopus embryos. (A,D,G) Lateral view of stage 18 embryos showing expression of (A) slug, (D) pdgfrα and (G) pdgf-a. (B,E,H) Lateral view of stage 24 embryos showing migrating NC expressing (B) twist, (E), pdgfrα and (H) pdgf-a. Scale bar: 1 mm. Grey stars indicate the eyes. (C,F,I) Sections of embryos shown in B,E,H, respectively. The line in B indicates the level of the section. Yellow dashed lines outline cephalic NC streams. (J) RT-PCR analysis of pdgf-a and pdgfrα expression in NC dissected from stage 18 embryos (premig. NC) and whole embryos along with Sox9 (NC marker), Sox2 (neural plate marker), brachyury (mesoderm marker), e-Keratin (epidermis marker) and ODC (control, ornithine decarboxylase). (K) Immunostaining for PDGFRα (green), Phalloidin (red) and DAPI (blue) in NC explants. Scale bars: 20 μm. (L) Western blot analysis of PDGFRα in NC dissected from control embryos, embryos treated with PDGF-A or embryos injected with PDGFRα MO. GADPH was used as a loading control. (M) Band intensity normalized to the loading control. Data are mean±s.d. of three independent experiments. AU, arbitrary units. ns, not significant; ***P<0.001.
Image published in: Bahm I et al.
© 2017. Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| snai2.L | slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 | X. laevis | Throughout NF stage 18 | premigratory neural crest cell |
| pdgfra.L | pdgfr2, pdgfr-alpha, pdgfralpha | X. laevis | Throughout NF stage 18 | premigratory neural crest cell |
| pdgfa.L | pdgf, pdgf-a | X. laevis | Throughout NF stage 18 | premigratory neural crest cell |
| twist1.L | twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist | X. laevis | Throughout NF stage 24 | neural crest migratory neural crest cell hyoid crest mandibular crest |
| pdgfra.L | pdgfr2, pdgfr-alpha, pdgfralpha | X. laevis | Throughout NF stage 24 | neural crest migratory neural crest cell |
| pdgfa.L | pdgf, pdgf-a | X. laevis | Throughout NF stage 24 | neural crest migratory neural crest cell |
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