Fig. 4 (left). Spatio-temporal expression of pou3f1 during Xenopus laevis development. (A) Expression of pou3f1 analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) at indicated stages of development. Average values from three independent experiments. (B) Whole mount in situ hybridization for pou3f1 at indicated stages of development. Early gastrula, vegetal view (a). Neurula, dorsal view (b,c). Tailbud, dorsal (d) and lateral (f) view. Tadpole, lateral view (g,i). Transverse sections at the level of the developing brain at tailbud stage (e) and at the level of the developing eye at tadpole stage (h). Abbreviations: e, eye; ep, epidermis; fb, forebrain; hb, hindbrain; mb, midbrain; nf, neural fold; np, neural plate; nt, neural tube; op, optic placode. (C) Expression of pou3f1 analyzed by RT-qPCR on dissected explants from tailbud-stage embryos, stage 25. Embryos were dissected either into ventral and dorsal halves or into tailbud, pronephros and head. Average values from three independent experiments. *: P < 0,05; **: P < 0,005, relative to the whole embryo.
Image published in: Cosse-Etchepare C et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, University of the Basque Country Press.
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