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Larger Image Fig. 1 Spatial and temporal expression of atf4 in Xenopus embryos (A-L) Whole mount in situ hybridization analysis data. (A-E) Anterior views. (F, G) Lateral views. (H) Anterior view of an embryo transversally dissected at the position indicated by the white dashed line in G. (I) Lateral view. (J) Ventral view of the embryo in I showing of the expression in the blood island. (K) Isolated gut. (L) Ventral view. ba, branchial arches; bi, blood island; cg, cement gland; ey, eye; hb, hindbrain; li, liver; mb, midbrain; opv, optic vesicle; ov, otic vesicle; pa, pancreas; pd, pronephric duct; sc, spinal cord; sd, part of stomach and duodenum. (M) RT-PCR analysis reveals the temporal expression profile of atf4 during Xenopus embryogenesis. UE, unfertilized egg. NC, negative control. Ornithine decarboxylase (ODC) was used as the loading control. Image published in: Liu JT et al. (2011) Copyright © 2011. OA ARTICLE under Creative Commons license.
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