Fig. 3. Expression of XSGLT1k examined by colorimetric in situ, FISH and FCIS methodologies. (A and B) XSGLT1k expression in stage 28/29 pronephros (red arrow). (C and D) XSGLT1k expression in stage 37 pronephros. (E) XSGLT1K expression in stage 37 embryo detected via FISH with an FITC-tyramide substrate. (F) Na+K+ATPase expression in the same embryo as panel F, detected with a Cy3-tyramide substrate. (G) Overlay of panels E and F. The proximal domain expressing XSGLT1k appears green. (H) XSGLT1k expression in a stage 30 embryo developed with a purple substrate. (I) The same embryo as shown in panel H but developed for Na+K+ATPase expression with an FITC-tyramide substrate. (J) FCIS overlay of panels H and I performed via the protocol described in Methods. The XSGLT1k domain appears blue, while the pronephric tubules in which it is not expressed appear orange.
Image published in: Zhou X and Vize PD (2004)
Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| slc5a9.L | sglt1k, sglt4, XSGLT1K | X. laevis | Throughout NF stage 28 | pronephric kidney pronephric mesenchyme |
| atp1a1.L | atp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPase | X. laevis | Throughout NF stage 29 and 30 | pronephric kidney pronephric duct pronephric tubule |
| slc5a9.L | sglt1k, sglt4, XSGLT1K | X. laevis | Throughout NF stage 37 and 38 | early proximal tubule pronephric kidney late proximal tubule |
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