Larger Image Fig. 4. Expression pattern of Raldh3 during Xenopus development. (A) Raldh3 expression as detected by whole-mount in situ hybridization of whole embryos (stage 10.25-31) or dissected neural retinas, without lens and pigmented epithelium (stage 59). cf, choroid fissure. Scale bar: 350 μm. (B) Raldh3 expression in transverse sections of stage 33 embryos after whole-mount in situ hybridization. From left to right, sections show expression at progressively posterior levels. op, olfactory placode; os, optic stalk. (C) Comparison of Pax2, Raldh3 and Vax2 expression in the optic cup in transversal sections of stage 33 embryos after whole-mount in situ hybridization. (D) Double in situ hybridizations of Rx1 with Raldh2, Shh or FGF8 on mid- to late neurula embryos shown from anterior view. The inset shows a double in situ hybridization of Rx1 with FGF8 on an early neurula embryo. Image published in: Lupo G et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue shh hedgehog, vhh-1, Xhh, X-SHH, xshh Xenopus Throughout neurula stage notochord floor plate aldh1a3 raldh3 Xenopus Throughout NF stage 10.25 blastopore lip upper blastopore lip aldh1a3 raldh3 Xenopus Throughout NF stage 11 dorsal aldh1a3 raldh3 Xenopus Throughout NF stage 12.5 dorsal chordal neural plate rax rax1, rax-a, rax-b, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 14 eye primordium optic vesicle fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 Xenopus Throughout NF stage 17 anterior neural fold aldh1a2 raldh-2, raldh2, XRaldh2 Xenopus Throughout NF stage 17 optic vesicle eye primordium anterior neural fold rax rax1, rax-a, rax-b, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 17 retina optic vesicle eye primordium fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 Xenopus Sometime during NF stage 17 to NF stage 18 anterior neural fold neuroectoderm midbrain-hindbrain boundary neural tube aldh1a3 raldh3 Xenopus Throughout NF stage 23 ventral optic vesicle midbrain-hindbrain boundary dorsal otic placode aldh1a3 raldh3 Xenopus Throughout NF stage 31 retina optic vesicle midbrain-hindbrain boundary otic vesicle otic placode cement gland ventral olfactory placode pax2 LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 Xenopus Throughout NF stage 33 and 34 retina ventral vax2 dres93, vax2-a, vax2-b, vax3, xvax2 Xenopus Throughout NF stage 33 and 34 retina optic stalk ventral aldh1a3 raldh3 Xenopus Throughout NF stage 33 and 34 ventral optic vesicle olfactory placode retina aldh1a3 raldh3 Xenopus Sometime during NF stage 59 to NF stage 60 ventral eye retina Image source: Published Permanent Image Page Printer Friendly View XB-IMG-23203