Fig. 4. Suppression of axonal outgrowth by hnRNP K MO in intact animals. Embryos unilaterally injected with control MO (A), hnRNP K MO1 (B,D-H) or hnRNP K MO2 (C) processed at stage 37/38 by whole-mount immunofluorescence. (A-C) Embryos injected with control MO (A), hnRNP K MO1 (B) or MO2 (C), and immunostained for NF-M. Broken lines separate injected (lower) from uninjected (upper) sides. (B,C) Arrowheads on the injected side indicate peripheral motor axons; arrowheads on the uninjected side indicate residual wispy peripheral axons. (A) View under the fluorescence dissecting microscope; (B,C) stacked confocal optical sections. (D1) Neuronal β-tubulin staining in neuronal perikarya and peripheral axons of spinal cord, uninjected side. (D2) Tubulin staining in perikarya and a few scattered fibers, injected side. (E1-G2) Peripherin immunostaining of head (E1,E2) and spinal cord (F1,F2,G1,G2). (E1,F1,G1) Uninjected side; (E2,F2,G2) injected side. (E1,E2) Trigeminal ganglion neuronal perikarya (upper arrowhead) and nerves (lower arrowhead). (F1,F2) Spinal cord with peripheral motor axons. (G1,G2) Spinal cord neuronal perikarya. (H) Spinal cord immunostained for HNK-1. Robust staining of neuronal perikarya and axons on the uninjected side (below the broken line); clusters of stained neuronal perikarya (arrowheads) and very few fibers on the injected side (above the broken line). Scale bars in D1,E2,F1 and G2 also apply to D2,E1,F2 and G1, respectively.
Image published in: Liu Y et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| prph.L | nef4, peripherin, XIF3 | X. laevis | Throughout NF stage 37 and 38 | neuron spinal cord ganglion trigeminal ganglion central nervous system hindbrain glossopharyngeal nerve vagus nerve trigeminal nerve facial nerve |
| b3gat1l.L | cd57, glcatp, glcuatp, hnk-1, hnk1, leu7, nk-1, nk1 | X. laevis | Throughout NF stage 37 and 38 | neuron spinal cord central nervous system peripheral nerve |
| nefm.L | nef3, nef-m, nefm-a, nefm-b, nf-m | X. laevis | Throughout NF stage 37 and 38 | brain spinal cord |
| tubb2b.S | NBT, neural beta-tubulin, NST, N-Tub, n-tubulin, ntubulin, Xn-tubulin | X. laevis | Throughout NF stage 37 and 38 | neuron spinal cord ganglion |
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