Figure 5. Rbmx is required for normal eye development and neuronal differentiation. A-H: Embryos injected unilaterally with Rbmx MO and cultured to stage 31 (A-D), stage 28 (E-F), or stage 35 (H). A,B: In situ hybridization for Xrx1. C,D: In situ hybridization for Pax6; red lines indicate Pax6 expression domain in normal forebrain (C), which is absent in the Rbmx MO injected side (D). E-H: Dorsal view of head and anterior trunk of Rbmx morphants. Injected side is to the right. E-H: In situ hybridization using antisense probe for En1 (E), Lbx1 (F), Lim1 (G), and Slug (H). Red arrowhead in F indicates Lbx1 expression in hypaxial muscle on the uninjected side. Yellow line in H indicates the midline and red arrow indicates melanocytes on the uninjected side. Notably Slug expression is absent in the neural tube and trunk on the injected side.
Image published in: Dichmann DS et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| en1.L | en-1, en1-a, en1-b, eng1, engrailed 1, engrailed-1, xen1 | X. laevis | Throughout NF stage 28 | midbrain-hindbrain boundary neural tube neuroectoderm |
| lbx1.L | hpx-6, hpx6, lbx1h | X. laevis | Throughout NF stage 28 | myotome mesoderm hindbrain spinal cord neural tube hypaxial muscle |
| lhx1.L | lim-1, Lim1, lim1, Xlim-1, Xlim1 | X. laevis | Throughout NF stage 28 | pronephric mesenchyme spinal cord mesoderm hindbrain neural tube neuroectoderm |
| rax.S | rax1, rax-a, rax-b, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A | X. laevis | Throughout NF stage 31 | retina pineal gland otic vesicle |
| pax6.L | an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 | X. laevis | Throughout NF stage 31 | eye retina lens placode diencephalon midbrain spinal cord forebrain hindbrain neural tube neuroectoderm |
| snai2.L | slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 | X. laevis | Throughout NF stage 35 and 36 | neural crest melanophore neural tube |
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