Fig. 3. Expression pattern of neural markers in embryos injected with XMeis3-AM RNA. Two-cell albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 50-100 pg of XMeis3âAM RNA. The red arrow delineates the dorsal midline. In all embryos, XMeis3-AM injection is on the left side. In all cases (except Fig. 3M), embryos are viewed dorsally; embryos are oriented anterior (top) to posterior (bottom). (A) In situ hybridization with otx2. Expression is expanded posteriorly on the XMeis3-AM injected side. The red lines delineate the AP extent of otx2 expression on the uninjected versus injected side. (B) In situ hybridization with otx2 and Krox20. otx2 expression is expanded posteriorly and Krox20 expression (blue arrows) is lost on the XMeis3-AM-injected side. The red lines delineate the AP extent of otx2 expression on the uninjected versus injected side. (C) In situ hybridization with otx2 and En2 (red). Expression of otx2 and En2 (blue arrows) is expanded posteriorly. (D) In situ hybridization with cpl-1 and En2 (red). cpl-1 expression is expanded posteriorly and En2 expression (blue arrow/uninjected side) is lost on the XMeis3-AM-injected side. The red lines delineate the AP extent of cpl-1 expression on the uninjected versus injected side. (E) In situ hybridization with XAG1; expression is expanded posteriorly and laterally on the XMeis3-AM injected side. (F) In situ hybridization with nrp1; expression is unchanged on the XMeis3-AM injected side. (G) In situ hybridization with Krox20 and HoxB9; Krox20 expression (blue arrows/uninjected side) is eliminated on the XMeis3-AM-injected side. HoxB9 expression is unchanged on the XMeis3-AM injected side. (H) In situ hybridization with XE10. XE10 expression is eliminated on the XMeis3-AM-injected side. (I) In situ hybridization with HoxB1. HoxB1 expression is eliminated on the XMeis3-AM injected side. (J) In situ hybridization with HoxB3. HoxB3 expression is eliminated on the XMeis3-AM-injected side. (K) In situ hybridization with En2 (red) and Krox20. En2 expression is pushed posteriorly to the r3/r4 boarder and Krox20 expression is pushed posteriorly to the r5/r7 boarder on the XMeis3-AM-injected side. (L) In situ hybridization with En2 and Krox20. En2 expression is pushed posteriorly to r3 on the XMeis3-AM-injected side. The blue arrow delineates the reduced Krox20 expression (fused stripes) on the injected side. (M) In situ hybridization with En2 (red) and Krox20. Expression of En2 and Krox20 (blue arrows/uninjected side) is eliminated on the XMeis3-AM-injected side. An anterior view of the embryo: dorsal (top) to ventral (bottom). (N) In situ hybridization with n-tubulin. n-tubulin expression is eliminated on the XMeis3-AM-injected side. The trigeminal neuron is marked by blue arrows on both sides.
Image published in: Dibner C et al. (2001)
Copyright © 2001. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hoxb3.S | hox2.7, hoxb-3, LOC108700804, LOC108702517 | X. laevis | Throughout NF stage 21 | hindbrain neuroectoderm rhombomere R5 rhombomere R6 |
| en2.L | En-2, en2-a, en2-b, eng2, engrailed 2, engrailed-2, engrailed homolog 2, maben, xen2 | X. laevis | Throughout NF stage 21 | neuroectoderm presumptive midbrain-hindbrain boundary |
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 21 | cranial neural crest neuroectoderm rhombomere R3 rhombomere R5 |
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 21 | neuroectoderm anterior anterior neural fold |
| hoxb1.L | hox-2.9, hoxb-1, XHox-b1 | X. laevis | Throughout NF stage 21 | midbrain neuroectoderm rhombomere R4 |
| ctsl.L | cathepsin L, catl, cpl-1, mep | X. laevis | Throughout NF stage 21 | anterior neural fold neuroectoderm pre-chordal neural plate |
| ag1.L | ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 | X. laevis | Throughout NF stage 21 | cement gland primordium |
| epha2.L | LOC100191024, XE10 | X. laevis | Throughout NF stage 21 | hindbrain neuroectoderm tail region rhombomere R4 |
| nrp1.L | A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 | X. laevis | Throughout NF stage 21 | central nervous system neuroectoderm |
| tubb2b.S | NBT, neural beta-tubulin, NST, N-Tub, n-tubulin, ntubulin, Xn-tubulin | X. laevis | Throughout NF stage 21 | neuron trigeminal ganglion rhombomere R2 |
| meis3.L | meis3-a, meis3-b, mrg2, XMeis3 | X. laevis | Throughout NF stage 21 | hindbrain spinal cord anterior |
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