Figure 2. xWnt6 mRNA is expressed during Xenopus organogenesis. A: Quantitative reverse transcriptase-polymerase chain reaction analysis shows the relative expression levels of Wnt6 mRNA. Wnt6 expression is normalized to histone H4 levels. B-K: xWnt6 mRNA localization determined by whole-mount in situ hybridization using a digoxigenin-labeled xWnt6-specific LNA probe. L-P: Control embryos hybridized with a digoxigenin-labeled nonspecific LNA control probe. Numerical figures indicate the developmental stage. Note, xWnt6 mRNA initially localizes to animal pole and neural fold tissues, then more generally throughout the ectoderm and later in epithelial tissues and in the somites, the developing eye and pronephros. ba, branchial arches; c, cloaca; ea, eye anlage; g, gills; h, heart; hm, hypaxial muscles; nf, neural folds; pa, pronephric anlage; pt, pronephric tubules; s, somites.
Image published in: Lavery DL et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 10 | ectoderm animal blastomere dorsal animal hemisphere |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 13 | ectoderm animal blastomere animal hemisphere |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Sometime during NF stage 18 to NF stage 19 | ectoderm anterior neural fold |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 23 | somite eye pronephric mesenchyme epidermis head |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 28 | ectoderm branchial arch fin |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 33 and 34 to NF stage 35 and 36 | ectoderm branchial arch fin pronephric nephron |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Sometime during NF stage 40 to NF stage 42 | pronephric nephron ectoderm branchial arch cloaca fin hypaxial muscle external gill |
| wnt6.S | wnt-6, XWnt-6, xWnt6 | X. laevis | Throughout NF stage 6 (32-cell) | animal blastomere ectoderm animal hemisphere |
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