Fig. 4. Whole-mount in situ hybridization of Xerl. (A) Dorsal view (st.15). Anterior is left. Arrowhead indicates posterior part of neural groove. (B) Anterior view of (A). Arrows show anterior part of neural plate. (C) Dorsal view (st.17). Anterior is left. Arrowhead indicates anteriorly bilateral expression. (D) Anterior view of (C). Arrowheads indicate anteriorly bilateral expression. Arrow indicates hypothalamus primodia. (E) Dorsal view (st.20). Anterior is left. Arrowhead indicates neural tube. (F) Anterior view of (E). Arrowheads indicate eye anlage and future midbrain. (G) Dorsal view (st.24). Anterior is left. (H) Anterior view of (G). Arrowheads indicate same region in (D,F). (I) Dorsal view (st.28). (J) Anterior view of (I). Arrowhead indicates midbrain expression. Arrow shows diencephalic expression. (K) Xpax6 expression (st.25). (L) Anterior view of (K). White arrowhead indicates blank of expression at midbrain. (M,Mâ²) Xpax6 expression (st.32). (N,Nâ²) Xtll expression (st.32). (O,Oâ²) Xerl expression (st.32). (MâO) Lateral view. (Mâ²âOâ²) Highly magnified dorsal view. (P,Q) Transverse section of the whole embryo stained with Xerl probe at st.32. (P) Midbrain. (Q) Lens vesicle. Arrows indicate remarkable expression at the lens equator zone. (R) In situ hybridization on the tissue sectioned at diencephalic level (st.32). Arrowheads indicate Xerl expression near the apical surface of ventricular zone. Arrow indicates Xerl expression of outer layer of neural retina. mb, midbrain; rm, rhombomere; mhb, mid-hindbrain boundary; te, telencephalon; di, diencephalon; ey, eye; gu, gut; lv, lens vesicle; nr, neural retina; dt, dorsal thalamus; ht, hypothalamus; ls, lens.
Image published in: Kuriyama S et al. (2000)
Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| crb2 | xerl | Xenopus | Throughout NF stage 15 | posterior neural groove pre-chordal neural plate |
| crb2 | xerl | Xenopus | Throughout NF stage 17 | anterior neural fold eye neural tube |
| crb2 | xerl | Xenopus | Throughout NF stage 20 | retina neural tube |
| crb2 | xerl | Xenopus | Sometime during NF stage 24 to NF stage 28 | eye brain forebrain midbrain |
| pax6 | an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 | Xenopus | Sometime during NF stage 25 to NF stage 32 | forebrain midbrain |
| crb2 | xerl | Xenopus | Throughout NF stage 32 | eye midbrain-hindbrain boundary dorsal midbrain retinal neural layer lens placode retina |
| nr2e1 | tailless, tlx, xTLL | Xenopus | Throughout NF stage 32 | forebrain midbrain |
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