Fig. 2. Scl marks and is essential for the emergence of adult haemangioblasts in the DLP. (A) Bar chart summarising the haematopoietic (magenta and red) and endothelial (green) expression hierarchy in the DLP reveals that adult haemangioblast programming takes place in four discrete transcriptional steps. Expression profiles were obtained by analysis of embryos subjected to whole-mount in situ hybridisation. Stages of development by morphological traits (NF) and hours post fertilisation (Hpf) are indicated at the top and are as indicated by Nieuwkoop and Faber (Nieuwkoop and Faber, 1967). (B) Expression analysis showing haematopoietic (Scl, Sox7 and Lyl1) and endothelial (Flt1, Flt4, Egr1, Elk3 and Egfl7) genes dependent on Scl for their expression in the DLP (arrows). (C) Expression analysis showing that haemogenic endothelium, as indicated by Runx1 and Gfi1a, fails to be established in the DA of Scl morphants. The DA is indicated by red arrows. (D) Expression analysis showing that the haematopoietic genes, Gata2, Lmo2 and Etv6; and the endothelial genes, Fli1, Flk1, Etv2 and Hex, in the DLP, as well as the expression of the growth factor, VegfA, are not dependent on Scl. The DLP is indicated by the red arrow. All embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. Embryos in B and D were analysed at stage 26, whereas those in C are stage 39 embryos. Numbers of embryos represented by each panel, out of the number analysed, are indicated in the top right corner. n, neurons, VBI, ventral blood island.
Image published in: Ciau-Uitz A et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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