Fig. 2. Whole-mount in situ hybridization using XMLP antisense RNA. Maternally expressed XMLP was present in cleave stages (A,B). Afterwards signals were found in the ectoderm and mesoderm of gastrula stages in (C,D). A sagittal section of stage11.5 is shown in (D). However, at neurula stage, signals were no longer evenly distributed and were restricted to neural ectoderm only (E,F,G). Subsequently, XMLP transcripts could be found in the neural folds at early tailbud stage and presumptive brain area only (H,I,M). The sagital section of (I) is shown in (J) and (K), while (L) shows a transversal section of (I). In later stages XMLP was expressed in ectoderm derivatives and pronephros during stage 27 to 34, (N,O,Q). (P) shows a transversal section of stage 34 (ac, archenteron; bt, blastocoel; bv, brain ventricle; ed, ectoderm; en, endoderm; hm, head mesenchyme; mc, mesencephalon; md, mesoderm; nc, notochord; ne, neural ectoderm; nt, neural tube; pn, pronephros; rc, rhombencephalon; sc, spinal chord).
Image published in: Zhao H et al. (2001)
Copyright © 2001. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| marcksl1.L | XMLP | X. laevis | Throughout NF stage 11.5 | ectoderm mesoderm |
| marcksl1.L | XMLP | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm |
| marcksl1.L | XMLP | X. laevis | Throughout NF stage 20 | neural tube posterior neural tube anterior neural tube eye eye primordium optic field cranial neural crest neural crest prechordal plate |
| marcksl1.L | XMLP | X. laevis | Sometime during NF stage 2 (2-cell) to NF stage 4 (8-cell) | early embryonic cell embryo |
| marcksl1.L | XMLP | X. laevis | Throughout NF stage 27 | spinal cord anterior dorsal lateral plate region hindbrain eye |
| marcksl1.L | XMLP | X. laevis | Throughout NF stage 33 and 34 | brain eye pronephric kidney central nervous system spinal cord intersomitic region pronephric duct pronephric nephron late proximal tubule late distal tubule pronephric tubule early distal tubule aortic arch hindbrain anterior lymph sac |
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