Fig. 3. Whole-mount in situ hybridization analysis of xTsg-2 and xTsg expression. (A,C) xTsg-2 expression at stage 27 and (B,D) stage 33 embryos showing expression in the branchial arches (ba), neural crest (nc), lateral plate mesoderm (lpm) and head mesenchyme (hm). Embryos in C and D were made transparent in Murray�s Clearing Solution (2:1 benzyl benzoate: benzyl alcohol) to visualize the expression of xTsg-2 localized to the midbrain-hindbrain border, the isthmus (is). D is a close up of the embryo in B. (E) Stage 27 and (F) stage 33 embryos stained for xTsg and cleared in Murray�s solution reveal expression in the dorsal eye (ey), heart anlage (he) and proctodeum (pro), as well as in the posterior wall (pw) of the tailbud. cg, cement gland; fb, forebrain; hb, hindbrain; pn, pronephros.
Image published in: Oelgeschläger M et al. (2004)
Copyright © 2004. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| twsg2.S | Tsg-2, Tsg2, xTsg-2, xTsg2 | X. laevis | Throughout NF stage 27 | lateral plate mesoderm pharyngeal arch lymphatic system anterior dorsal lateral plate region hindbrain head mesoderm |
| twsg1.S | tsg, twisted gastrulation, twsg, twsg1-a, twsg1-b | X. laevis | Throughout NF stage 27 | proctodeum tail bud cardiac mesoderm eye forebrain |
| twsg2.S | Tsg-2, Tsg2, xTsg-2, xTsg2 | X. laevis | Throughout NF stage 33 and 34 | head neural crest lateral plate mesoderm pronephric kidney ophthalmic vein middle cerebral vein pharyngeal arch branchial arch spinal cord roof plate anterior dorsal lateral plate region hindbrain lymphatic system lymph vessel |
| twsg1.S | tsg, twisted gastrulation, twsg, twsg1-a, twsg1-b | X. laevis | Throughout NF stage 33 and 34 | eye tail bud posterior wall of neurenteric canal post-anal gut rectal diverticulum unspecified |
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