Larger Image Fig. 5. Spatial expression of Xoom during oogenesis and embryogenesis. Whole-mount in situ hybridization with digoxigenin-labeled Xoom RNA probe was performed in albino oocytes and embryos. (A) Oocyte stage I to VI (left to right). Animal pole is up. (B) Lateral view of early blastula (st. 7). Animal pole is up. (C) Animal view of gastrula (st. 10). (D) Dorsal view of early neurula (st. 15). Zygotic expression occurs in the dorsoanterior region (arrow). (E) Dorsal view of late neurula (st. 19). Expression of Xoom is localized in both sides of the neural tube (arrow) and upper space of the eye vesicles (arrowheads). (F) Anterior view of late neurula (st. 19). Expression of Xoom is localized in both sides of neural tube (arrow) and upper space of eye vesicles (arrowheads). (G) Transverse section at the trunk region of late neurula (st. 19). nt, neural tube; nc, notochord; so, somite. Xoom specifically expresses in neural crest cells around the neural tube. (H) Lateral view of tadpole (st. 35). (I) High magnification of cleared tadpole head. Intense expression of Xoom is observed in the optic vesicle (arrow) and the otic vesicle (arrowhead). Image published in: Hasegawa K et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher.
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