Larger Image Fig. 3. Whole mount xGAT-1 expression in the developing Xenopus embryo. Insets show a stage-matched embryo hybridized with an antisense GAD probe. A: xGAT-1 expression is first detected in the spinal cord during late neurula stages (stage 21). B: xGAT-1 signal is present in the hindbrain and forebrain by stage 23. C: By stage 26, expression increases in the spinal cord and can be observed in all parts of the developing brain. DâF: xGAT-1 expression continues to strengthen during tailbud stages (D, stage 28; E, stage 32), and by swimming tadpole stages (F, stage 40) is present at high levels throughout the central nervous system and eye. G: Higher magnification of the head of a stage 33 embryo showing distinct domains of xGAT-1 expression in the forebrain, midbrain, and hindbrain. H: Dorsal view of a tailbud stage embryo (stage 32). fb, forebrain; hb, hindbrain; mb, midbrain; ol, olfactory placodes; sc, spinal cord. Scale bar 1 mm in AâH. Image published in: Li M et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher.
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