Fig. 6. Whole-mount in situ hybridization of xPN-1, xNuc and xEDJ. Embryos are shown in animal (A), dorsal (B,D), lateral (C,H,I) or anterior view (E-G). (A-C) Xenopus Proteinase Nexin-1 (xPN-1). (A) Four-cell stage embryo showing high level of maternal transcripts. (B) Late neurula. Note expression in the notochord (n) and ear placode (e). (C) Early tail bud stage with additional expression domains in the lens (le) and ectoderm of the branchial arches (ba). (D-F) Xenopus Nucleobindin (xNuc). (D) Midgastrula showing expression in the notochord (n). (E) Early neurula with additional signal in the cement gland (cg). (F) Late neurula. Note strong expression in the cement gland and hatching gland (hg). (G-I) Xenopus ER-associated DNAJ chaperone (xEDJ). (G) Late neurula with expression in cement gland (cg) and hatching gland (hg). (H) Late neurula depicting faint signal on the ventral side (arrowhead). (I) Early tail bud stage. Note expression in the pronephros (pn) and in the hatching gland in the dorsal head.
Image published in: Pera EM et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nucb1.S | calnuc, nuc, nucleobindin | X. laevis | Throughout NF stage 11 | presumptive axial mesoderm |
| nucb1.S | calnuc, nuc, nucleobindin | X. laevis | Throughout NF stage 13 | notochord axial mesoderm cement gland primordium |
| serpine2.L | gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 | X. laevis | Throughout NF stage 17 | notochord axial mesoderm preplacodal ectoderm neural plate |
| dnajb11.L | EDJ | X. laevis | Sometime during NF stage 18 to NF stage 20 | hatching gland cement gland cement gland primordium |
| nucb1.S | calnuc, nuc, nucleobindin | X. laevis | Throughout NF stage 20 | cement gland hatching gland |
| serpine2.L | gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 | X. laevis | Throughout NF stage 26 | lens notochord cranial neural crest branchial crest otic placode otic vesicle |
| dnajb11.L | EDJ | X. laevis | Throughout NF stage 28 | hatching gland pronephric kidney |
| serpine2.L | gdn, glia-derived nexin, pn-1, pn1, Protease nexin-1, xpn-1 | X. laevis | Throughout NF stage 4 (8-cell) | animal blastomere animal animal pole |
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