Figure 1. Xenopus appl1 is expressed in the developing pancreas and SD of tadpole stage embryos. A-I: Whole mount in situ hybridization data for appl1 expression. A, B: Blastula and gastrula embryos bisected after whole mount in situ hybridization. C, D: Anterodorsal view. E: Lateral view. F: Dorsal view. G-I: Whole mount in situ hybridization on dissected whole guts. J: Western blot revealed temporal expression of appl1 during Xenopus embryogenesis. Beta-actin served as a protein loading control. K: RT-PCR analysis of appl1 temporal expression. UE, unfertilized egg. N, negative control. Ornithine decarboxylase (ODC) was used as the RNA loading control.
Image published in: Wen L et al. (2010)
Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 11 | animal hemisphere blastocoel roof marginal zone |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 17 | neural plate anterior neural fold pre-chordal neural plate neuroectoderm |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 21 | neuroectoderm neural plate neural crest cranial neural crest mandibular crest hyoid crest branchial crest anterior neural tube anterior placodal area |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 32 | eye branchial arch otic vesicle pronephric kidney pharyngeal arch mandibular arch hyoid arch spinal cord brain olfactory region pronephric duct glomus forebrain |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 35 and 36 | central nervous system brain forebrain midbrain hindbrain spinal cord pronephric kidney cephalic nerve |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 41 to NF stage 43 | pancreas stomach duodenum endoderm alimentary system |
| appl1.L | appl, dip13alpha | X. laevis | Throughout NF stage 7 | animal hemisphere animal cap animal cap inner layer animal cap outer layer |
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