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Larger Image Fig. 1. Expression pattern of Mst1 and Mst2 in Xenopus tropicalis development. (A) RT-PCR analysis revealed the temporal expression patterns of Mst1 and Mst2. RNA was extracted from Xenopus tropicalis embryos at the stages indicated above each lane. ODC were used as internal controls. ODC(-), RT-PCR without reverse transcriptase in ODC reaction. e, unfertilized egg. (B-P) Whole-mount in situ hybridization analysis of Mst1 and Mst2. (B-H) Localized expression pattern of Mst1. (B�) The anterior view corresponding to (B). (C�-F�) The ventral view corresponding to C-F. (I) Hemi-sectioning of a stage-18 embryo revealed detailed localization of Mst1 in the aVBI. (I�) High magnification view of the boxed region in (I). (J-M,O,P) Localized expression pattern of Mst2. (J�-M�,O�) The ventral view corresponding (J-M, P,O). (N) Transverse sectioning of a stage-28 embryo revealed detailed localization of Mst2 in the pVBI. (N�) High magnification view of the boxed region in (N). Scale bars, 100 +m. View direction is indicated in the upper right of each panel. Developmental stage is indicated in the lower right. Abbreviations: ba, branchial arch; du, duodenum; in, intestine. Image published in: Nejigane S et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, University of the Basque Country Press.
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