Figure 5. Expression domains of selected molecular marker genes validates the pronephric segmentation model. Whole-mount in situ hybridizations of stage 35/36 Xenopus embryos were performed. Lateral views of whole embryos (left panels), enlargements of the pronephric region (middle panels), and color-coded schematic representations of the segment-restricted expression domains (right panels) are shown. (a) cldn8: intermediate tubule (IT)2. Note that the expression levels are low. Arrowheads indicate the proximal and distal boundaries of the cldn8 expression domain. (b,d) cldn14 and cldn16: intermediate tubule, distal tubule (DT)1. (d) cldn19: nephrostomes (arrowheads), intermediate tubule. (e) clcnk: intermediate tubule, distal tubule, connecting tubule. Note that the dotted staining pattern localizes to cells of the epidermis. The localization of the clcnk expression domains has previously been reported [22] and is shown here for comparative purposes. (f) kcnj1: IT1, distal tubule, connecting tubule. The arrowhead indicates the location of IT2, which fails to express kcnj1. (g) calb1: connecting tubule. The arrowhead indicates the proximal boundary of the expression domain. Expression is highest in the most distal parts of the connecting tubule.
Image published in: Raciti D et al. (2008)
Copyright © 2008 Raciti et al. Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cldn8.4 | claudin-8 | X. tropicalis | Throughout NF stage 35 and 36 | distal tubule pronephric kidney |
| cldn14.L | claudin-14, DFNB29 | X. laevis | Throughout NF stage 35 and 36 | distal tubule pronephric kidney early distal tubule late distal tubule head |
| cldn16.L | claudin-16, LOC108718049, PCLN1 | X. laevis | Throughout NF stage 35 and 36 | distal tubule pronephric kidney early distal tubule late distal tubule |
| clcnkb.L | ClC-k, clc-kb, clckb, clcnka, clk-k2, LOC108703568, xClC-k | X. laevis | Throughout NF stage 35 and 36 | distal tubule pronephric kidney pronephric duct early distal tubule late distal tubule epidermis |
| kcnj1.L | kir1.1, MGC68935, ROMK, romk1 | X. laevis | Throughout NF stage 35 and 36 | distal tubule pronephric kidney pronephric duct early distal tubule late distal tubule |
| calb1.L | calb, calb1-a, calb1-b, calbindin | X. laevis | Throughout NF stage 35 and 36 | head brain eye pharyngeal region otic vesicle posterior pronephric duct retina |
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